I.  REVIEW OF THE GENERAL PROCEDURE FOR THE ENRICHMENT, ISOLATION AND IDENTIFICATION OF BACTERIA as outlined in the lab manual:

ENRICHMENT AND ISOLATION						PURE CULTURE WORK
SOURCE MATERIAL		BROTH ENRICHMENT		PLATING FOR ISOLATION		STOCK CULTURES		CHARACTERIZATION & IDENTIFICATION
"Consider inoculum: what organisms may or may not be present. "May pre-treat inoculum, e.g., by heat-shocking.		"Usually is selective. "May be skipped altogether.		"Usually selective or selective-differential – but not always!!
		Throughout procedure, appropriate media and incubation conditions must be considered.

II.  A "WORKSHEET" TO HELP US ORGANIZE AND SUMMARIZE THE SPECIFIC FACTORS that allow isolation of the various types of microorganisms in these experiments:

	11A Streptomyces	11B Purple "non-sulfur" photosynthetic bacteria	11C Bacillus	11D N2-fixing bacteria
Special properties of desired organism that can be exploited to help us "sort it out" from others.
Likely source material (habitat of organism or where the organism is a significant contaminant).
Treat sample to enhance isolation?
Need to use enrichment?
Factors to consider for the enrichment & plating media:* "Selective by restriction of nutrients or addition of selective agents? "Special sources of C, N, energy?	(Why is 2nd plating medium an all-purpose medium?)	**
Special incubation conditions?		**
Detectable features of the desired organism that help us in picking and identifying it.		**
Any special, additional tests on the isolates? (That is, tests not essential to identification to the genus level.)

  *  For the desired type of organism, the medium/media must include the required elements (including trace elements) and whatever growth factors are needed. Note that these organisms are all heterotrophs and organotrophs. (A review of Appendix D in the manual is useful.)

**  See question 11B in the compilation of old quiz questions. How would we want to grow the purple non-sulfur photosynthetic bacteria to maximize their detection and minimize growth of other organisms – as phototrophs or as chemotrophs?